ggplot2 条形图的子集 data.frame

Question

I have the following data:

    Splice.Pair  proportion
1         AA-AG 0.010909091
2         AA-GC 0.003636364
3         AA-TG 0.003636364
4         AA-TT 0.007272727
5         AC-AC 0.003636364
6         AC-AG 0.003636364
7         AC-GA 0.003636364
8         AC-GG 0.003636364
9         AC-TC 0.003636364
10        AC-TG 0.003636364
11        AC-TT 0.003636364
12        AG-AA 0.010909091
13        AG-AC 0.007272727
14        AG-AG 0.003636364
15        AG-AT 0.003636364
16        AG-CC 0.003636364
17        AG-CT 0.007272727
...       ...   ...

I want to get a barchart visualising the proportion of each splice pair but only for splice pairs that have a proportion over, say, 0.004. I tried the following:

nc.subset <- subset(nc.dat, proportion > 0.004)
qplot(Splice.Pair, proportion, data=nc.dat.subset,geom="bar", xlab="Splice Pair", ylab="Proportion of total non-canonical splice sites") + coord_flip();

But this just gives me a bar chart with all splice pairs on the Y-axis, except that the splice pairs that were filtered out are missing bars.

I have no idea what is happening to allow all categories to still be present:s

Answer 1

What's happening is that Splice.Pair is a factor. When you subset your data frame, the factor retains it's levels attribute, which still has all of the original levels. You can avoid this kind of problem by simply wrapping your subsetting in droplevels :

nc.subset <- droplevels(subset(nc.dat, proportion > 0.004))

More generally, if you dislike this kind of automatic retention of levels with factors, you can set R to store strings as character vectors rather than factors by default by setting:

options(stringsAsFactors = FALSE)

at the beginning of your R session (this can also be passed as an option to data.frame as well).

EDIT

Regarding the issue of running older versions of R that may lack droplevels , @rcs points out in a comment that the method for a single factor is very simple to implement on your own. The method for data frames is only slightly more complicated:

function (x, except = NULL, ...) 
{
    ix <- vapply(x, is.factor, NA)
    if (!is.null(except)) 
        ix[except] <- FALSE
    x[ix] <- lapply(x[ix], factor)
    x
}

But of course, the best solution is still to upgrade to the latest version of R.

Answer 2

Check whether Splice.Pair is a factor. If that's the case, use droplevels() to remove the levels that are no longer used to resolve your problem.

nc.subset <- subset(nc.dat, proportion > 0.004)
nc.subset$Splice.Pair <- droplevels(nc.subset$Splice.Pair)
qplot(Splice.Pair, proportion, data=nc.dat.subset,geom="bar", xlab="Splice Pair", ylab="Proportion of total non-canonical splice sites") + coord_flip();

You may be able to incorporate droplevels into qlot , but that's for you to find you:-)