Why i get this error when i publish asp.net app on iis?

Question

I want to publish my asp.net application to iis,but when i run the application i get this error:

i exert this link:
enter link description here

but i get this error now:

Answer 1

It looks like you are using integrated authentication for your SQL connection and the user that the AppPool is running under has not been granted access to the database. If you intended to use integrated authentication, you should add the domain login corresponding to the AppPool id to your database and grant it access. If not, then update your connection string with the correct SQL credentials - probably in your release config transform - and redeploy.