[英]How to export pixel coordinates of point objects from an image (with imagej?)
I am on a project where I am interested in the spacing & distribution of point objects in an microscope image. 我在一个项目中,我对显微镜图像中点对象的间距和分布感兴趣。
So the image consists of darker, circular small dots from a rather complex background. 因此,图像由来自相当复杂背景的较暗的圆形小点组成。 It would be nice to do this with an feature extraction script automatically some day, but for now I am just hand-picking the points... Because I trust my own eyes :) 有一天会自动使用功能提取脚本来完成此操作会很好,但是现在我只是手工挑选要点...因为我相信自己的眼睛:)
I looked through a couple different softwares and so far I liked imagej, which has a multi-point select feature. 我浏览了几种不同的软件,到目前为止,我喜欢imagej,它具有多点选择功能。 But I couldn't figure out after selecting the points by hand, how to export the coordinates. 但是我无法在手动选择点后弄清楚如何导出坐标。 All I need is the X's and Y's for each point (for about a hundred points for each image). 我需要的是每个点的X和Y(每个图像大约一百个点)。
Any pointers? 有指针吗?
Or if you have alternative ideas on how to do this, suggest me another program (matlab?), or even point me to other useful readings, etc... 或者,如果您对如何执行此操作有其他想法,请建议我另一个程序(matlab?),或者甚至指向其他有用的阅读材料,等等。
Thanks immensely! 非常感谢!
Though it doesn't seem to be the same process you're after there is something similar written in ImageJ for analysing 'Dot Blots' 虽然看起来似乎与您使用的过程不同,但ImageJ中有类似的东西可以用来分析“斑点印迹”
http://image.bio.methods.free.fr/dotblot.html http://image.bio.methods.free.fr/dotblot.html
It has a number of methods for identifying the dots and can also export the results as a CSV. 它有多种识别点的方法,也可以将结果导出为CSV。 The code itself is available at the bottom of the above page so you should be able to work with it if you're interested in automation. 该代码本身在上一页底部提供,因此,如果您对自动化感兴趣,则应该可以使用它。
OK. 好。 With some help I discovered that I can do this with just CTRL-M in imagej... 在一些帮助下,我发现可以使用imagej中的CTRL-M来完成此操作...
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