I have a file which I want to take all the lines which starts with CDS and a line below. This lines are like:
CDS 297300..298235
/gene="ENSBTAG00000035659"
I found this in your site:
open(FH,'FILE');
while ($line = <FH>) {
if ($line =~ /Pattern/) {
print "$line";
print scalar <FH>;
}
}
and it works great when the CDS is only a line. Sometimes in my file is like
CDS join(complement(416559..416614),complement(416381..416392),
complement(415781..416087))
/gene="ENSBTAG00000047603"
or with more lines in the CDS. How can I take only the CDS lines and the next line of the ID??? please i need your help! Thank you in advance.
Assuming the "next line" always contains /gene=
, one can use the flip-flop operator .
while (<>) {
print if m{^CDS} ... m{/gene=};
}
Otherwise, you need to parse the CDS line. It might be sufficient to count parens.
my $depth = 0;
my $print_next = 0;
while (<>) {
if (/^CDS/) {
print;
$depth = tr/(// - tr/)//;
$print_next = 1;
}
elsif ($depth) {
print;
$depth += tr/(// - tr/)//;
}
elsif ($print_next) {
print;
$print_next = 0;
}
}
You need to break the input into outdented paragraphs. Outdented paragraphs start a non-space character in their first line and start with space characters for the rest.
Try:
#!/usr/bin/env perl
use strict;
use warnings;
# --------------------------------------
my $input_file = shift @ARGV;
my $para = undef; # holds partial paragraphs
open my $in_fh, '<', $input_file or die "could not open $input_file: $!\n";
while( my $line = <$in_fh> ){
# paragraphs are outdented, that is, start with a non-space character
if( $line =~ m{ \A \S }msx ){
# don't do if very first line of file
if( defined $para ){
# If paragraph starts with CDS
if( $para =~ m{ \A CDS \b }msx ){
process_CDS( $para );
}
# delete the old paragraph
$para = undef;
}
}
# add the line to the paragraph,
$para .= $line;
}
close $in_fh or die "could not close $input_file: $!\n";
# the last paragraph is not handle inside the loop, so do it now
if( defined $para ){
# If paragraph starts with CDS
if( $para =~ m{ \A CDS \b }msx ){
process_CDS( $para );
}
}
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