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Python - Trying to Get My Sound File To Play On Startup of Application

I have used the following code and I am not sure why it is not working.

from PythonCard import model

playSound = 1

class MainWindow(model.Background):
    pass

app = model.Application(MainWindow)
app.MainLoop()

if playSound == 1:
    s = Sound()
    s.read('start.wav')
    s.play
    playSound = 0

使用括号来调用函数:

s.play()

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